Confocal Laser Scanning Microscopy (CLSM)
Our laboratory features a homebuilt confocal laser scanning microscope (CLSM) equipped to perform a wide range of imaging and spectroscopic techniques. Additionally, it is combined with the light microscope for widefield imaging. This versatile system supports high-resolution imaging and quantitative measurements, making it a powerful tool for studying dynamic processes and molecular interactions in complex systems.
CLSM for Advanced Imaging and Analysis
The CLSM is optimized for various applications, including fluorescence correlation spectroscopy (FCS), raster image correlation spectroscopy (RICS), fluorescence lifetime imaging (FLIM), image correlation spectroscopy (ICS), spatiotemporal image correlation spectroscopy (STICS). Key features of the system include:
- Excitation versatility: A range of pulsed lasers covering key excitation wavelengths for fluorescence imaging.
- High-sensitivity detection: Equipped with avalanche photodiodes (APDs) for precise single photon counting.
- Live-cell imaging: PECON incubation chamber for long-term live-cell imaging at 37°C and 5% CO₂.
- RICS/ICS/STICS capabilities: Advanced correlation analysis of spatiotemporal dynamics in living cells and tissues.
- FLIM capabilities: Lifetime measurements with picosecond time resolution for studying molecular interactions and environmental dynamics.
- FCS and FCCS support: For quantifying molecular concentrations, diffusion rates, and interaction kinetics in solution or in live cells.
- Widefield reference imaging: A range of continuous wave lasers are installed to quickly locate cells of interest.
Latest Applications
This system serves as a central platform for exploring various dynamic and structural phenomena in biological and physical systems. Recent applications include:
- Mapping intracellular diffusion and molecular interactions using RICS and cross-correlation RICS.
- High-resolution imaging of subcellular structures and dynamic processes.
- Quantifying diffusion and transport mechanisms in living cells at different time scale via RICS and STICS.
- Diffusion and interaction studies in solution using FCS and FCCS.
- Lifetime-based studies of molecular environments with FLIM.