PAM - PIE analysis with MATLAB

Schrimpf W*, Barth A*, Hendrix J, Lamb DC
PAM: A Framework for Integrated Analysis of Imaging, Single-Molecule, and Ensemble Fluorescence Data.
Biophys. J 114, 2018 [doi link]

Introduction

PAM is a software package for quantitative analysis of fluorescence microscopy and spectroscopy data, with a focus on experiments using pulsed interleaved excitation. PAM combines many advanced fluorescence methods, including:

• Fluorescence fluctuation techniques (FCS, FCCS, Pair-Correlation)
• Single-molecule Förster resonance energy transfer (smFRET) in solution with multiparameter fluorescence detection (MFD)
• Fluorescence lifetime imaging microscopy (FLIM) using the phasor approach
• Image analysis and fluctuation imaging (ICS, RICS, tICS, N&B)

The software is hosted in the repository at https://www.gitlab.com/PAM-PIE/.

Compiled versions (Nov. 2024) can be downloaded using the following links:
  Windows | MacOS

The manual can be found at http://pam.readthedocs.io.